Cell Manipulation and Cytometry

BSCRC Flow Cytometry Core BSCRC Flow Core Staff: Jeffrey Calimlim, Felicia Codrea, Jessica Scholes, BSCRCFlowCore@mednet.ucla.edu (310) 825-4408 TLSB 3045. https://stemcell.ucla.edu/flow-cytometry-core. The Flow Cytometry Core provides the UCLA research community with flow cytometry instrumentation and expertise in cell analysis and cell sorting services.  The instruments use Fluorescence-Activated Cell Sorting, or FACS, technology, which enables users to analyze cell populations, isolate cells with special characteristics and characterize their biochemical, genomic and epigenetic properties.



Cellular Bioenergetics Core Laurent Vergnes lvergnes@ucla.edu (310) 267-2741 695 Charles E. Young Drive South http://people.healthsciences.ucla.edu/institution/core-detail?group_id=1838053 Measure of cellular respiration and glycolysis from cells, mitochondria, tissues, worm and yeast.

UCLA Flow Cytometry Core Flow Cytometry Core  Zoran Galic, PhD (310) 825-1043 Factor Building 12th Floor. http://cyto.mednet.ucla.edu/home.html The core laboratory provides instrumentation and technical and professional assistance for performing laser-based flow cytometric analysis and cell sorting. The facility also offers training in basic flow cytometry principles and in the operation of analytic cytometers. A second course teaches nucleic acid analysis and measurement of proliferation by flow cytometry as well as hands-on instrument set-up and use of the specialized DNA analysis software.

Immune Assessment Core Maura Rossetti mrossetti@mednet.ucla.edu (310) 983-3993 1000 Veteran Ave., Rehab Center A-538 http://pathology.ucla.edu/body.cfm?id=651 Provides both standardized and customizable multi-parameter flow cytometry, immunoassays and functional assays for assessing various immune cell functions, including T cells, B cells, NK cells, monocytes and granulocytes. Validated assays include several immunophenotyping panels, allo- and virus-specific T cell activation, T and B cell proliferation, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. In addition, it provides singleplex and multiplex immunoassays on ELISA and Luminex platforms.

bioenergetics Immuno/BioSpot Core Brent Gordon brgordon@ucla.edu (310) 206-8252 Factor Building 12-541 & BSRB 188F http://people.healthsciences.ucla.edu/institution/core-detail?group_id=36215 The Immuno/BioSpot Core specializes in assays related to the detection, quantification, and qualification of biological spot assays and single-cell related analyses, including ELISPOT, colony counting, plaque assays, FluoroSpot, cell viability tests, apoptosis tests, in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes, histochemistry stains, genotoxicity assays, multi-color intracellular/surface quantification, etc.

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