Cell Manipulation and Cytometry
Cellular Bioenergetics Core
695 Charles E. Young Drive South
Measure of cellular respiration and glycolysis from cells, mitochondria, tissues, worm and yeast.
The core laboratory provides instrumentation and technical and professional assistance for performing laser-based flow cytometric analysis and cell sorting. The facility also offers training in basic flow cytometry principles and in the operation of analytic cytometers. A second course teaches nucleic acid analysis and measurement of proliferation by flow cytometry as well as hands-on instrument set-up and use of the specialized DNA analysis software.
Provides both standardized and customizable multi-parameter flow cytometry, immunoassays and functional assays for assessing various immune cell functions, including T cells, B cells, NK cells, monocytes and granulocytes. Validated assays include several immunophenotyping panels, allo- and virus-specific T cell activation, T and B cell proliferation, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. In addition, it provides singleplex and multiplex immunoassays on ELISA and Luminex platforms.
Factor Building 12-541 & BSRB 188F
The Immuno/BioSpot Core specializes in assays related to the detection, quantification, and qualification of biological spot assays and single-cell related analyses, including ELISPOT, colony counting, plaque assays, FluoroSpot, cell viability tests, apoptosis tests, in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes, histochemistry stains, genotoxicity assays, multi-color intracellular/surface quantification, etc.